Oligonucleotide & Primer Resuspension Calculator

Calculate the volume of TE buffer or sterile water needed to resuspend lyophilized DNA/RNA oligonucleotides to target concentrations.

Input Parameters

Instant Real-time Calculation

Oligo Quantity on Spec Sheet (nmol)

Desired Stock Concentration (µM; Standard 100 µM)

Result Chemistry

Buffer Volume to Add

-- microliters (µL)

Export calculation:

Calculation Logic:

Calculates reconstitution volume for synthetic DNA/RNA primers. Adding 10× the nmol quantity in microliters of buffer yields exactly 100 µM stock.

⚡ 100% Free Tool • Instant Calculation • No Sign-Up

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Understanding Oligonucleotide & Primer Resuspension Calculator & Formula

Mathematical Formula: Buffer Volume (µL) = (Oligo Amount in nmol / Desired Concentration in µM) × 1000

The Oligonucleotide & Primer Resuspension Calculator on CalculatorScale.com is engineered to deliver instant, mathematically verified calculations conforming strictly to standard institutional equations. Whether planning long-term investments, managing household budgets, tracking clinical fitness targets, analyzing academic genetics, calculating veterinary dosages, or converting dimensional units, every calculation updates dynamically in real time without lag.

Every formula used in this tool adheres to certified international standards, including World Health Organization biometric metrics, veterinary clinical guidelines, NIST physical conversion constants, and standard biological equations. Results are computed entirely client-side inside your browser with complete data privacy, ensuring your sensitive numbers and statistics never leave your device.

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Quick 100 µM Oligo Preparation Rule

Spec Sheet Yield (nmol)	Desired Molarity	Buffer Volume to Add	Resulting Concentration
25.0 nmol	100 µM	250 µL	100 µM (100 pmol/µL)
35.4 nmol	100 µM	354 µL	100 µM (100 pmol/µL)
50.0 nmol	100 µM	500 µL	100 µM (100 pmol/µL)
10.0 nmol	10 µM (Working)	1,000 µL	10 µM (10 pmol/µL)

How to Use the Oligonucleotide & Primer Resuspension Calculator

1 Locate total nanomoles (nmol) printed on your primer tube spec sheet.
2 Select target concentration (100 µM is standard for master stocks; 10 µM for working stocks).
3 Read exact microliters (µL) of sterile TE buffer or nuclease-free water to pipette into the vial.

FAQ & Help 1 question